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Water resource design and projects need sufficient information and data processes to ensure proposed hydraulic structures are successful. Many hydrological studies have no observed data for discharges in the basins, and one of the most commonly used concepts for previous hydrological studies is the flow duration curve. This work shows a new approach to estimate the flow duration curve in basins for which there is no information. The proposal presents a calibrated equation for each of the six regions in the state of Antioquia, in the northwest of Colombia. Veering from the traditional approach, it includes the non-exceedance probability. The statistical analysis conducted shows a fitting error of less than 13% for all regions in Antioquia except Urabá, which has an error of 44%.